Penetapan Kadar Simultan Vitamin B Kompleks dalam Sediaan Tablet dan Sirup secara Spektrofotometri Ultraviolet dengan Metode Absorption Factor dan Area Under Curve

Abstract

The various forms of pharmaceutical dosage available on the market consist of a combination of two or more effective substances in one preparation, one of which is a supplement containing vitamins B1, B2, B3, B6, and B12. Quality inspection of pharmaceutical preparations in determining the content of effective substances is an important part so that reliable analytical methods, tools, and operational costs are relatively cheaper, and easy to implement, to provide results with good accuracy and precision. The ultraviolet spectrum of vitamin B1 has a maximum wavelength of 246 nm, vitamin B2 267 nm, vitamin B3 262 nm, vitamin B6 290 nm, and vitamin B12 361 nm. Simultaneous determination of vitamins B1, B2, B3, B6, and B12 content by classical ultraviolet spectrophotometry is difficult because the wavelengths are adjacent. The development of ultraviolet spectrophotometry methods, including absorption factor and area under curve methods, has been carried out on various types of dosage forms. This study aimed to develop ultraviolet spectrophotometry with absorption factor and area under curve methods to determine the content of vitamins B1, B2, B3, B6, and B12 in tablet and syrup dosage simultaneously. Research was conducted by selecting the type of solvent, water, ethanol, and methanol in an optimized manner. An ultraviolet spectrophotometry method by absorption factor and area under curve then tested its validity based on validation parameters of linearity, LOD, LOQ, accuracy, and precision. The method was applied to determine the content of vitamins B1, B2, B3, B6, and B12 in tablet and syrup forms. The results showed that methanol was the best solvent for analysis. Application of absorption factor method in determination of content was done at λ1 361 nm, λ2 291 nm, λ3 269 nm, λ4 262 nm, and λ5 239 nm respectively for vitamins B12, B6, B2, B3, and B1, area under curve method in determination of content was done at λ 233-243 nm, λ 263-273 nm, λ 252-262 nm, λ 285-295 nm, and λ 355-365 nm for vitamins B1, B2, B3, B6, and B12 respectively. Based on the results of the study, it can be concluded that the absorption factor and area under curve methods, ultraviolet spectrophotometry can be used to determine the content of vitamins B1, B2, B3, B6, and B12 in tablet and syrup and have fulfilled the validation requirements.