Efektivitas Pemberian Nanoherbal Gagatan Harimau (Paraboea leuserensis B.L.Burtt) terhadap Histologi, Ekspresi Caspase 3 dan Caspase 10 pada Hati dan Otot Tikus (Rattus norvegicus L.) Setelah Latihan Fisik Maksimal

Abstract

This study aims to analyze Histology, Expression of Caspase 3 and Caspase 10 in Rat Liver and Muscle Cells After Maximum Physical Exercise Given of Gagatan Harimau Nanoherbal (Paraboea leuserensis B.L.Burtt). The research was conducted from April to June 2023. The research locations were the Animal Physiology Laboratory and Experimental Animal House, Department of Biology, FMIPA USU, PT. Synergy of Indonesian Nanotech, South Tangerang, Banten, and the Anatomic Pathology Laboratory, Faculty of Medicine, University of North Sumatra, Medan. Treatment consists of control group, maximum physical exercise, maximum physical exercise + Vitamin C at a dose of 2 mg/Kg Body Weight, maximum physical exercise + Gagatan Harimau nanoherbal with concentration 100, 125 and 150 mg/Kg BW. Data obtained from each observation parameter were recorded and presented in table and figure form. The data were analyzed using SPSS software version 25 with significant differences at 5%. The results of the study showed all treatments (p<0.05) in the expression of caspase 3 and caspase 10 in rat liver and muscle. At dose of 150 mg/Kg BW can reduce the expression of caspase 3 and caspase 10 in rat liver and muscle. Induction of Gagatan Harimau leaf nanoherbal as an antioxidant in male rats given swimming physical exercise showed improvements in liver and muscle histology and morphology. A dose of 150 mg/Kg BW of nanoherbal Gagatan Harimau leaves was the best dose because it showed cell regeneration close to the normal treatment group.