Pembuatan 2-Etilheksil Laurat dari Esterifikasi Asam laurat dan 2-Etilheksanol Pada Perbandingan Rasio Mol dan Berat Enzim Lipase Menggunakan Radiasi Mikrowave

Abstract

The production 2-Ethylhexyl Laurate from esterification reaction of Lauric acid with 2-ethylhexanol with lipase enzyme as catalyst under microwave irradiation has been conducted. The parameters studied in this study were the effect of lipase enzyme catalyst weight and molar ratio of lauric acid to 2-ethylhexanol. The confirmation of 2-ethylhexyl laurate formation was studi using FT-IR and LC-MS spectrophotometer while the conversion was determined by alkalimetric titration. The formation of ester groups from the resulting 2-ethylhexyl laurate is characterized by the presence of a peak at wave number 1714,6 cm-1 showing stretching vibrations of carbonyl groups (C=O) indicating the presence of esters and supported by the presence of asymmetric and symmetric C-O stretching vibrations at wavenumbers of 1230 cm-1 and 1036,2 cm-1 . The LC-MS spectra showed the fragmentation pattern identical to 2-ethylhexyl laurate with ration m/z 297, 257, and 234. The maximum conversion of 93.50 ± 1.98% for lipase enzyme catalyst weight parameter was achieved under reaction condition of 3% catalyst weight, 1:2 moles ratio, 10 minutes reaction time and 20% microwave power. For the parameter of moles ratio, the maximum conversion of 97.65 ± 1.18% was obtained at a ratio of 1:3, 3% catalyst weight, 10 minutes reaction time, and 20% microwave power.