Perbandingan Sampel Urin dan Apusan Endoserviks untuk Mengidentifikasi Chlamydia Trachomatis dengan Metode Polymerase Chain Reaction pada Wanita Pekerja Seksual

Abstract

Introduction : Chlamydia trachomatis (CT) often causes Sexually Transmitted Infections (STIs) in developed and developing countries. In general, CT infection is asymptomatic, especially in women. To prevent further complications of CT infection, screening tests are needed, especially in women with high risk as in Female Sex Workers (FSW). Polymerase Chain Reaction (PCR) is the recommended method for identifying CT. Objective: To know the comparison between urine samples and endocervical swabs to identify CT by PCR method in FSW. Method: This is a cross sectional descriptive study. Subjects of this study are 58 urine samples and 58 endoservical swasb from 58 FSW. First catch urine and endocervical swab are collected. The samples from the urines and endocervical swabs were tested by PCR. Result : Of the 58 FSWs with the most characteristic are 25-29 years old and most 34 years old (36.2%), completed junior high school/equal (63.8%) and duration of work as FSW more than 12 months (91.4%). PCR method for Chlamydia trachomatis from urine sample were positive (22.4%). PCR method for Chlamydia trachomatis from endocervical swab were positive (27.6%). Comparison of Chlamydia trachomatis PCR between urine samples and endocervical swabs (22.4% : 27.6%). Chlamydia trachomatis PCR from urine sample and endocervical swab were obtained (Positive urine, positive endocervical) (20.7%), (Positive urine, negative endocervical) (1.7%), (Negative urine, positive endocervical) (6.9%) and (Negative urine, negative endocervical) (70.7%). Conclusion : Comparison urine samples and endocervical swabs to Identify Chlamydia trachomatis by PCR method are (22.4% : 27.6%).

Pendahuluan : Chlamydia trachomatis (CT) sering menyebabkan Infeksi Menular Seksual (IMS) baik di negara maju maupun negara berkembang. Pada umumnya infeksi CT bersifat asimtomatis terutama pada wanita. Untuk mencegah komplikasi lebih lanjut infeksi CT diperlukan uji skiring terutama pada wanita yang mempunyai resiko tinggi seperti pada Wanita Pekerja Seksual (WPS). Polymerase Chain Reaction (PCR) merupakan metode yang direkomendasikan untuk mengidentifikasi CT. Tujuan : Untuk mengetahui perbandingan sampel urin dan apusan endoserviks untuk mengidentifikasi CT dengan metode PCR pada WPS. Metode : Penelitian deskriptif, cross sectional. Subjek penelitian sebanyak 58 sampel urin dan 58 apusan endoserviks dari 58 orang WPS. Dilakukan penampungan urin pancaran pertama dan pengambilan apusan endoserviks. Kemudian dilakukan PCR Chlamydia trachomatis dari sampel urin dan apusan endoserviks. Hasil : Dari 58 orang WPS dengan karakteristik usia terbanyak kelompok 25-29 tahun dan >34 tahun masing-masing 36,2%, dengan pendidikan terbanyak SMP sederajat (63,8%) dan durasi terlama bekerja >12 bulan (91,4%). PCR Chlamydia trachomatis sampel urin didapat hasil positif (22,4%) dan negatif (77,6%). PCR Chlamydia trachomatis apusan endoserviks didapat hasil positif (27,6%) dan negatif (72,4%). Perbandingan PCR Chlamydia trachomatis antara sampel urin dan apusan endoserviks (22,4% : 27,6%). PCR Chlamydia trachomatis sampel urin dan apusan endoserviks didapat (Urin positif, endoserviks positif) (20,7%), (Urin positif, endoserviks negatif) (1,7%), (Urin negatif, endoserviks positif) (6.9%) dan (Urin negatif, endoserviks negatif) (70,7%). Kesimpulan : Perbandingan PCR Chlamydia trachomatis sampel urin dan apusan endoserviks adalah 22,4% : 27,6%.