Analisis Asam Lemak Galur Mutan Kedelai (Glycine max L. Merril) Generasi M5 Dengan Metode Kromatoragfi Gas
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Date
2020Author
Siringoringo, Ivana Juliandri
Advisor(s)
lubis, Khairunnisa
Damanik, Revandyiskandar m
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Show full item recordAbstract
This study aims to determine the composition of the fatty acid content of
the M5 generation mutant soybean lines using the gas chromatography method.
This research was conducted at the Central Laboratory of the Faculty of
Agriculture, University of North Sumatra, Medan and Indonesian Oil Palm
Research Institute, Medan from August to October 2020. Soybean oil was
obtained from 12 mutant lines, 1 parent and 1 comparison variety which were
extracted using the Soxhlet method with Hexan (Pa) solvent, while the analysis of
the composition of fatty acid content was carried out by the gas chromatography
method. The analysis results obtained 10 types of composition of fatty acid
content in soybeans and the ones that dominate are linoleic fatty acids (53%) and
oleic fatty acids (51,8%). Linoleic and oleic fatty acids are polyunsaturated fatty
acids and are classified as essential fatty acids. Penelitian ini bertujuan untuk mengetahui komposisi kandungan asam
lemak yang terdapat pada galur mutan kedelai generasi M5 dengan metode gas
kromatografi. Penelitian ini dilakukan di Laboratorium Sentral Fakultas Pertanian
Universitas Sumatera Utara, Medan dan Laboratorium PPKS (Pusat Penelitian
Kelapa Sawit), Medan pada Agustus s/d Oktober 2020. Minyak kedelai diperoleh
dari 12 galur mutan, 1 tetua dan 1 varietas pembanding yang telah di ekstraksi
menggunakan metode Soxhlet dengan pelarut Hexan (Pa), sementara analisis
komposisi kandungan asam lemak dengan metode gas kromatografi. Hasil analisis
diperoleh 10 jenis komposisi kandungan asam lemak pada kacang kedelai dan
yang mendominasi adalah asam lemak linoleat (53%) dan asam lemak oleat
(51,8%). Asam lemak linoleat dan oleat merupakan asam lemak tidak jenuh
berantai banyak dan tergolong asam lemak esensial.
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