Efek Ekstrak Etanol Daun Pirdot (saurauia vulcani k.) Terhadap Kadar Gula Darah dan Mikrostruktur Pankreas Mencit (mus musculus l.) Model Diabetes Tipe 2

Abstract

Research on the effect of pirdot (Saurauia vulcani K.) leaf ethanol extract on blood glucose levels and pancreas microstructure in type 2 diabetic mice (Mus musculus L.) model has been done from June until October 2020. Pirdot leaf is used traditionally as an anti-diabetic, but its effect on lowering blood sugar and repairing pancreatic beta cells still needs to have researched. The research was performed using completely randomized design with five treatments and five replications. The treatments consisted of normal control (K-) (without treatment), positive control (K +) (induced of STZ 50 mg / kg BW), treatment group (P1): STZ (50 mg / kg BW) + pirdot leaf ethanol extract 100 mg / kg BW, treatment group (P2): STZ (50 mg / kg BW) + pirdot leaf ethanol extract 200 mg / kg BW, treatment group (P3): STZ (50 mg / kg BW) + pirdot leaf ethanol extract 300 mg / kg BW. Blood glucose levels were measured on day 0, after injection of Streptozotocin (STZ), then measurements were taken once every 5 days, until day 35. Pancreatic organ preparations are made by paraffin method and phloxine staining. The results showed that pirdot leaf extract was able to reduce blood sugar levels at a dose of 200 mg / kg BW, could improve langerhans island diameter and increase the number of langerhans island beta cells.

Penelitian mengenai efek ekstrak etanol daun pirdot (Saurauia vulcani K.) terhadap kadar gula darah dan mikrostruktur pankreas mencit (Mus musculus L.) model diabetes tipe 2 telah dilakukan dari bulan Juni hingga Oktober 2020. Daun pirdot digunakan secara tradisional sebagai obat anti diabetes, akan tetapi efeknya terhadap penurunan gula darah dan perbaikan sel-sel beta pankreas masih perlu diteliti. Metode penelitian menggunakan rancangan acak lengkap (RAL) dengan 5 perlakuan dan 5 ulangan. Perlakuan terdiri dari kontrol normal (tanpa perlakuan), kontrol positif yang diinduksi 50 mg/kg BB streptozotocin (STZ) dan tiga kelompok yang disuntik streptozotocin dan diberi ekstrak etanol daun pirdot dengan dosis 100, 200, atau 300 mg/kg BB. Seluruh hewan uji diberi pakan tinggi lemak dan minum air secara ad libitum. Kadar gula darah diukur pada hari ke-0, setelah penginduksian streptozotocin, kemudian dilakukan pengukuran setiap 5 hari sekali, sampai hari ke 35. Preparat organ pankreas dibuat dengan metode parafin dan pewarnaan phloxine. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun pirdot mampu menurunkan kadar gula darah pada dosis 200 mg/kg BB, dapat memperbaiki diameter pulau langerhans dan meningkatkan jumlah sel beta pulau Langerhans.