Perbedaan Kadar Enzim Katalase pada Wanita Menopause dan Wanita Usia Reproduktif

Abstract

Objective: to determine difference between catalase enzyme levels in menopausal and reproductive aged women. Method: this analytical case control analytical study, was conducted in the H. Adam Malik General Hospital from September 2014. Samples were consecutively sampled and included menopausal women aged 45-60 years and 20-40 year old reproductive aged women. Menopausal subjects were asked to fill an L-MMPI (Minnesota Multiphasic Personality Inventory) scale, followed by a 29 question MENQOL (Menopause-Spesific Quality of Life Questionnaire) scale. Blood sampling was performed from all respondents to determine catalase enzyme levels. Data were then descriptively analysed to observe frequency and characteristic distributions, mean values, and standard deviations and numeric data. Intervariable discrepancies were analysed using the t-independent test with CI of 95% (p<0,05). Results: 32 menopausal and 32 reproductive aged women were dominated by 56,2% 31-40 year old reproductive aged women, whereas 50% of menopausal subjects were aged 50-54 year old. Catalase enzyme mean levels in menopausal and reproductive aged women were 12.24 mU/ml dan 105.94 ± 22.37 mU/ml, respectively, revealing a significant difference between the two groups (p<0,05). Results from 29 MENQOL questions revealed mean variations for each complaint. Menopausal complaints were dominated by sexual symptoms, followed by physical, vasomotor, and psychosocial symptoms with mean values of 3.89 ± 1.65; 2.74 ± 1.9; 2.39 ± 1.34; and 2.36 ± 1.12, respectively. Conclusion: Catalase enzyme levels significantly different between menopausal and reproductive aged women.

Tujuan: mengetahui perbedaan kadar enzim katalase pada wanita menopause dan wanita usia reproduktif. Metode: merupakan penelitian analitik dengan pendekatan case control, dilakukan di RSUP. H. Adam Malik Medan mulai bulan September 2014. Sampel adalah wanita menopause usia 45-60 tahun dan wanita usia reproduktif usia 20–40 tahun dikumpulkan secara consecutive sampling. Subjek menopause mengisi skala L-MMPI (Minnesota Multiphasic Personality Inventory) kemudian skala MENQOL (Menopause-Spesific Quality of Life Questionnaire) dengan 29 pertanyaan. Dilakukan pengambilan sampel darah terhadap peserta penelitian untuk mengetahui kadar enzim katalase. Data di analisa secara deskriptif untuk melihat distribusi frekuensi dari karakteristik, nilai rerata (mean), dan standard deviasi serta data numerik. Untuk menganalisa perbedaan antar variabel dilakukan uji t-independent dengan derajat kepercayaan 95% (p<0,05). Hasil: ada 32 orang wanita usia reproduktif dan 32 orang wanita menopause dengan mayoritas responden wanita usia reproduktif berusia 31–40 tahun 56,2% sedangkan wanita menopause berusia 50-54 tahun 50%. Rerata kadar enzim katalase pada wanita menopause 155,53 ± 12,24 mU/ml dan 105,94 ± 22,37 mU/ml pada wanita usia reproduktif yang secara statistik terdapat perbedaan bermakna antara kedua kelompok tersebut (p < 0,05). Dari 29 pertanyaan MENQOL terdapat variasi nilai rerata tiap keluhan menopause. Keluhan menopause dengan rerata tertinggi ada pada domain gejala seksual 3,89 ± 1,65 diikuti gejala fisik 2,74 ± 1,9; gejala vasomotor 2,39 ± 1,34; dan gejala psikososial 2,36 ± 1,12. Kesimpulan: terdapat perbedaaan yang bermakna antara kadar enzim katalase pada wanita usia menopause dan wanita usia reproduktif.