Ekspresi Gen Hoxa 10 Jaringan Endometrium Penderita Endometriosis yang Infertil

Abstract

Objective : To find the difference of HOXA10 gene expression in endometrial tissue of infertile women with endometriosis and normal endometrium. Method : This is an analytic study with cross sectional approach. 40 subjects who fulfilled the inclusion criteria were obtained, 20 subjects with endometriosis and 20 subjects without endometriosis. Endometrial tissue from infertile women with endometriosis and from normal women were obtained using endometrial biopsy. Immunohistochemistry examination was then performed to evaluate HOXA10 expression. This study was performed in Obstetric and Gynecology Department of Adam Malik General Hospital Medan from March to April 2015. The obtained data was then analyzed statistically. Result : Majority of subjects from endometriosis group were from the age group of 31-40 years old with 13 subjects (65%). While the majority of subjects from non endometriosis group were from the age group 41-50 years old with 15 subjects (75%). Based on parity, majority of the subject from endometriosis were nulliparous with 19 subjects (95%). From the non endometriosis group, subjects with parity of 1-3 were 11 subjects (55%). Based on HOXA10 expression intensity, 14 subjects (70%) showed no expression, expression of +1 with 6 subjects (30%). While from non endometriosis group, expression of +3 were found in 13 subjects (65%), and expression of +2 were found in 7 subjects (35%), p<0,05. Conclusion : There is a significant difference of HOXA10 gene expression in endometrial tissue of subject with endometriosis and without endometriosis.

Tujuan : Untuk mengetahui perbedaan ekspresi gen HOXA10 pada jaringan endometrium wanita infertil dengan endometriosis dan endometrium normal. Metode : Penelitian ini merupakan studi analitik dengan pendekatan potong lintang. 40 subjek yang memenuhi kriteria inklusi dikumpulkan, 20 subjek dengan endometriosis dan 20 subjek tanpa endometriosis. Jaringan endometrium pada wanita infertil dengan endometriosis dan pada wanita normal didapatkan dengan biopsi endometrium. Pemeriksaan immunohistokimia dilakukan untuk mengevaluasi ekspresi HOXA10. Penelitian ini dilakukan di Departemen Obstetri dan Ginekologi RSUP HAM Medan dari bulan Maret sampai April 2015. Data yang telah diperoleh kemudian dianalisa secara statistik. Hasil : Sebagian besar subjek dari kelompok endometriosis berasal dari kelompok umur 31-40 tahun didapati sebanyak 13 subjek (65%). Dimana sebagian besar subjek yang berasal dari kelompok non endometriosis umur 41- 50 tahun didapati sebanyak 15 subjek (75%). Berdasarkan kriteria paritas, sebagian besar subjek dengan endometriosis nullipara didapati sebanyak 19 subjek (95%). Dari kelompok non endometriosis, sebagian besar subjek dengan paritas 1-3 didapati sebanyak 11 subjek (55%). Berdasarkan intensitas ekspresi HOXA10, 14 subjek (70%) menunjukkan tidak ada ekspresi, ekspresi +1 didapati 6 subjek(30%). Kelompok non endometriosis dengan ekspresi +3 ditemukan pada 16 subjek (65%), dan ekspresi +2 ditemukan pada 7 subjek (35%), p<0,05 Kesimpulan : Didapati perbedaan yang signifikan dari subjek dengan ekspresi gen HOXA10 pada jaringan endometrium dan tanpa endometriosis.