Kadar Vascular Endothelial Growth Factor (Vegf) Serum pada Wanita dengan Endometriosis

Abstract

Introduction: Angiogenesis has a role in growth and development of endometriosis. The objective this study was to determine the level VEGF serum women with endometriosis compared to non endometriosis. Methods: this comparative analytical case control designed study obtained 33 endometriosis and 33 non-endometriosis cases through consecutive random sampling based on inclusion and exclusion criterias. Stage of endometriosis was assessed based on the ASRM (American Society of Reproductive Medicine), and pain severity was assessed using the VAS (Visual Analog scale). Endometriosis and peritoneum tissues of non endometrosis subjects were obtained by laparoscopy or laparatomy, afterwhich the diagnosis was confirmed histopathologically. VEGF serum was measured using the reagan kit (Human VEGF Immunoassay) (Quantikine®; R&D system, Minneapolis, MN, USA) by Prodia Laboratory. Results: VEGF serum levels of endometriosis signifacantly differed from non endometriosis subjects with mean values 436.64±176.87 vs 182.22±57.23, respectively and p value < 0.0001. Based on age groups, the severity of pain, VEGF serum levels of the endometriosis group did not differ to the non-endometriosis group. Results showed that the means Moderate-Severe stage was higher than Minimal-Mild stage with means 464.51±175.91 vs 280.54 ±74.05. Conclusion: Level of VEGF in serum of women with endometriosis signifacantly differed from non endometriosis.

Pendahuluan: Angiogenesis mempunyai peranan dalam pertumbuhan dan perkembangan endometriosis. Penelitian ini bertujuan untuk mengetahui perbedaan kadar VEGF serum wanita yang menderita endometriosis dibandingkan dengan non endometriosis Metode: Penelitian ini merupakan analisis komparatif dengan rancangan case control. Dengan consecutive random sampling didapatkan 33 kasus endometriosis dan 33 non endometriosis yang sesuai dengan kriteria inklusi dan ekslusi. Stadium endometriosis dinilai berdasarkan ASRM (American Society of Reproductive Medicine), dan derajat nyeri dinilai dengan menggunakan VAS (Visual Analog Scale). Jaringan endometriosis dan jaringan peritoneum non endometrosis dari laparoskopi maupun laparatomi diperiksa histopatologi untuk konfirmasi diagnosis. VEGF serum diperiksa dengan reagen kit (Human VEGF Immunoassay) (Quantikine®; R&D system, Minneapolis, MN, USA) oleh Laboratorium Klinik Prodia. Hasil: Ditemukan perbedaan bermakna kadar VEGF serum endometriosis dibandingkan non endometriosis dengan rerata 436,64±176,87 vs 182,22±57,23 dengan nilai p < 0,0001. Berdasarkan umur, derajat nyeri tidak ditemukan perbedaan bermakna antara kadar serum VEGF endometriosis dibandingkan kelompok non endometriosis. Hasil penelitian ini menunjukkan bahwa kadar VEGF serum pada stadium sedang-berat lebih tinggi dibandingkan dengan stadium minimal-ringan dengan rerata adalah adalah 464,51±175,91 vs 280,54 ±74,05. Kesimpulan: terdapat perbedaan yang bermakna secara signifikan kadar VEGF serum wanita dengan endometriosis dibandingkan non endometriosis.