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    Inkorporasi Asam Askorbat pada Pembentukan Selulosa Bakteri dengan Menggunakan Acetobacter Xylinum

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    Date
    2010
    Author
    Yusak, Yuniarti
    Advisor(s)
    Marpaung, Harlem
    Wirjosentono, Basuki
    Slalahi, Jansen
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    Abstract
    Incorporation of ascorbic acid at forming bacterial cellulose by using Acetobacter xylinum. Coconut water as a substance, use for making bacterial cellulose thrugh phosphate pentose pathway with juice Acetobacter xylinum bacteria and it was used for making starter. And starter of coconut water used to synthesize bacterial cellulose . Making of bacterial cellulose by ascorbic acid various 0, 0,5, 1,0 ,1,5 and 2,0 gram from cellulose for fourteen days. The product was produced and then analyzed with qualitative and quantitative. The optimum result can be obtained by the addition of 1 g ascorbic acid which the thickness was 0,935 cm, ash concentration resut 1,57 %, water concentration 86,73 %,fiber concentration 3,55 %. Analysis of vitamin ( ascorbic acid) by iodometry method and the result got increasingly ascorbic acid concentration and reached vitamin C with high concentration. The analysis to be continued with infrared spectrophotometric to analysis absorption peak of carbonyl group and the surface of particle of bacterial cellulose was analyzed by scanning electrone microscopy(SEM). From the result of research can produce a sheap product, natural, and healthy used for a supplement food.
     
    Air kelapa sebagai bahan dasar, digunakan untuk pembuatan selulosa bakteri, melalui jalur pentosa fosfat dengan menggunakan bakteri Acetobacter xylinum dan bakteri ini, mula-mula digunakan untuk pembuatan starter. Starter air kelapa inilah yang kemudian, digunakan untuk mensintesis selulosa bakteri. Pembuatan selulosa bakteri dengan menvariasikan asam askorbat dari 0,0,5, 1,0, 1,5 dan 2,0 g ,membentuk selulosa bakteri selama 14 hari.Produk yang dihasilkan kemudian dianalisa secara kualitatif dan kuantitatif. Pada penambahan asam askorbat 1 g , didapat hasil yang optimum dimana didapat ketebalan sebesar 0,935 cm, kadar abu 1,57 %, kadar air 86,73 % dan kadar serat 3,55 %, dan kadar vitamin C makin bertambah jumlah vitamin C, kadar vitamin C makin meningkat.Analisa dilanjutkan dengan spektrofotometri inframerah untuk mengetahui terjadinya inkorporasi dengan adanya pergeseran gugus karbnilnya, .Kemudian dianalisa dengan SEM untuk mengetahui partikel-partikel permukaan dari selulosa bakteri tersebut.Hasil penelitian ini, diharapkan dapat menghasilkan produk yang murah,sehat dan alami untuk digunakan sebagai tambahan makanan .

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    http://repositori.usu.ac.id/handle/123456789/43812
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    • Doctoral Dissertations [102]

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    Repositori Institusi Universitas Sumatera Utara (RI-USU)
    Universitas Sumatera Utara | Perpustakaan | Resource Guide | Katalog Perpustakaan
    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV