Uji Potensi Bakteri Kitinolitik dalam Menghambat Pertumbuhan Rhizoctonia solani Penyebab Rebah Kecambahpada Kentang Varietas Granola

Abstract

A study on assay of potential of chitinolytic bacterial isolates to inhibit the growth of Rhizoctonia solanicausal agent of damping-off on potato plants has been conducted in Laboratory of Observation Pest and Disease, Medan Johor, UPT. Protection of Crops and Holticulture 1 and Laboratory of Microbiology, Department of Biology, Faculty of Mathematic and Natural Science ofSumatera Utara University, Medan. Two out of six isolatesof chitinolytic bacteria showed to inhibitof R. solanigrowth. The most effective isolates to inhibit the growth of R. solani was Enterobacter sp. BK15 and Bacillus sp. BK13 inhibited the fungal growth with inhibition zone of 2.05 and 2.35 cm, respectively. These two isolates also showed to be able to reduce fungal infection by 31.25% and 37.5%,respectively.

Penelitian tentang uji potensi bakteri kitinolitik dalam menghambat pertumbuhan Rhizoctonia solani penyebab rebah kecambah pada tanaman kentang telah dilakukan di Laboratorium Pengamatan Hama dan Penyakit, Medan Johor, UPT Balai Proteksi Tanaman Pangan dan Hortikultura 1, Medan dan Laboratorium Mikrobiologi, Departemen Biologi, FMIPA, Medan. Dua dari enam isolat bakteri yang telah diujikan memiliki potensi paling efektif dalam menghambat pertumbuhan R. solani. Isolat bakteri yang paling efektif menghambat pertumbuhan R. solani adalah Enterobacter sp. BK15 dan Bacillus sp. BK13dengan zona hambat berturut-turut yaitu 2,05 cm dan 2,35 cm. Dua isolat ini menunjukkan pengurangan serangan jamur secara berturut-turut yaitu 31,25% dan 37,5%.