dc.contributor.advisor | Hastuti, Liana Dwi Sri | |
dc.contributor.author | Putri, T. Qisti Aqila | |
dc.date.accessioned | 2022-07-11T08:35:50Z | |
dc.date.available | 2022-07-11T08:35:50Z | |
dc.date.issued | 2022 | |
dc.identifier.uri | https://repositori.usu.ac.id/handle/123456789/49474 | |
dc.description.abstract | Crop failure due to root-root nematode (NPA) attack on tomato plants can reach 29%.
The use of chemicals in the long term can cause negative impacts on the environment,
therefore biocontrol agent in the form of nematophagus fungi is needed to be an
appropriate alternative in overcoming the problems caused by M. hapla. This study
aims to determine the potential of the fungi Trichoderma yunnanense (J2) from Lake
Toba and Monacrosporium spp. (LKTS1) from Lau Kawar in inhibiting the growth of
nematodes in vitro and reducing the attack of M. hapla on tomato plants in vivo. This
study used a completely randomized design, in which the two fungi isolates were
treated with nematodes. Observations were made every 12, 24, 36, and 72 hours with
3 repetitions which saw a reduction in nematode growth. In the bioassay test, 6
treatments were given, namely K+, K-, KBF, J2, LKTS1, and J2+LKTS1. Observations
were made at 7, 14, 21, and 30 days with 4 repetitions. The indicators taken as data
were the number of vermiform nematodes, the number of root swellings, the number
of root knots, plant height, crown height, root height, crown fresh weight and dry
weight, root fresh weight and dry weight. In vitro test results, J2 reduced nematode
growth by 99.30% and LKTS1 reduced nematode growth by 99.80%. The results of
the bioassay test, the most effective treatment in suppressing root cavities in the
bioassay test is the combination treatment of isolates J2 and LKTS1 (J2+LKTS1)
which has the highest percentage of 98.2%, while the treatment of isolate J2 alone
inhibits 97.8% and LKTS1 alone inhibits by 98.1%. | en_US |
dc.description.abstract | Gagal panen akibat serangan Nematoda Puru Akar (NPA) pada tanaman tomat dapat
mencapai 29%. Penggunaan bahan kimia dalam jangka waktu yang lama dapat
menyebabkan dampak negatif bagi lingkungan, sehingga diperlukan agen biokontrol
berupa jamur nematofagus terhadap nematoda untuk menjadi salah satu alternatif yang
tepat dalam mengatasi masalah yang ditimbulkan oleh Meloidogyne hapla. Penelitian
ini bertujuan untuk mengetahui potensi jamur Trichoderma yunnanense (J2) asal
Danau Toba dan Monacrosporium spp. (LKTS1) asal Lau Kawar dalam menghambat
pertumbuhan nematoda secara in vitro dan mengurangi serangan Meloidogyne hapla
pada tanaman tomat secara in vivo. Penelitian ini menggunakan Rancangan Acak
Lengkap, pada uji in vitro kedua isolat jamur diberikan nematoda. Pengamatan
dilakukan setiap 12, 24, 36, dan 72 jam dengan 3 kali pengulangan yang melihat
pengurangan pertumbuhan nematoda. Pada uji bioassay diberikan 6 perlakuan, yaitu
K+, K-, KBF, J2, LKTS1, dan J2+LKTS1. Pengamatan dilakukan pada 7, 14, 21, dan
30 hari dengan 4 kali pengulangan. Indikator yang diambil sebagai data adalah jumlah
nematoda vermiform, jumlah bengkak akar, jumlah puru akar, tinggi tanaman, tinggi
tajuk, tinggi akar, berat basah dan kering tajuk, berat basah dan kering akar. Hasil uji
in vitro, J2 mengurangi pertumbuhan nematoda sebesar 99,30% dan LKTS1
mengurangi pertumbuhan nematoda sebesar 99,80%. Hasil uji bioassay, perlakuan
paling efektif dalam menekan puru akar pada uji bioassay adalah perlakuan kombinasi
isolat J2 dan LKTS1 (J2+LKTS1) memiliki persentase tertinggi sebesar 98,2%,
sedangkan perlakuan isolat J2 sendiri menghambat sebesar 97,8% dan LKTS1 sendiri
menghambat sebesar 98,1%. | en_US |
dc.language.iso | id | en_US |
dc.publisher | Universitas Sumatera Utara | en_US |
dc.subject | Nematode-Trapping Fungi | en_US |
dc.subject | M. hapla | en_US |
dc.subject | T. Yunnanense | en_US |
dc.subject | Monacrosporium spp.. | en_US |
dc.title | Efektivitas Jamur Nematofagus Isolat Sumatera Utara sebagai Biokontrol Meloidogyne Hapla pada Tanaman Tomat (Solanum lycopersicum L.) | en_US |
dc.type | Thesis | en_US |
dc.identifier.nim | NIM170805077 | |
dc.description.pages | 79 Halaman | en_US |
dc.description.type | Skripsi Sarjana | en_US |