Pengaruh Curcuminoid terhadap Ekspresi Calcineurin (Cn) pada Fibroblas Koklea Rattus Norvegicus yang Diberi Pajanan Bising

Abstract

Background: Numerous epidemiological and experimental studies have been carried out so far, from which empirical evidences were obtained and various theories were suggested to elucidate a series of noise-induced hearing loss (NIHL) process at the molecular level. Objective: This study was conducted to demonstrate curcuminoid as the safe and effective therapeutic agent in order to treat the damage of cochlear fibroblasts, which may lead to NIHL viewed from the expression of calcineurin (Cn). Methods: The samples were 24 Rattus norvegicus divided into 4 groups. Group 1: control group; group 2: noise (+); group 3: noise (+), 50 mg/day curcuminoid ( + ); group 4: noise ( + ), 100 mg/day curcuminoid ( + ). The noise exposure dose given was 100 dB SPL for 2 hours in 2 weeks and the curcuminoid were administered orally in 2 weeks. This study used curcuminoid derived from Curcuma longa Linnaeus (Turmeric) with curcuminoid content levels [28.1 ± 1.0]% w/w compared to standard. All samples were examined for the expression of Cn in cochlear fibroblasts by immunohistochemistry method. Results: The results obtained showed significant differences for the expression of Cn (p < 0.05) in all groups. Curcuminoid proved to be potentially effective in the treatment for the damage of cochlear fibroblasts regarding the decreased expression of Cn.