Keragaman Bakteri Peraian Hutan Mangrove Belawan dan Potensinya dalam Proses Dekolorisasi Limbah zat Warna

Abstract

Mangrove forest is an ecosystem that has a high diversity of microorganisms with the ability to degrade toxic compounds, especially in the mangrove area in Belawari, Medan City, North Sumatra which is often exposed to waste carried by rivers This study aims to determine the diversity of bacteria in the water.~ of the Belawan mangrove f orest and ils po1ential in the process of decolorizing dye-was1e. This study was initiated by isolation of bacteria using Nutrient Agar media (+1% NaCl) f rom water samples taken at 3 locations in the waters of the Belawan mangrove forest, purification, characterization, molecular identification and detennination of the potential for decolorization of dye-waste. There are 29 isolates were obtaindfrom the three locations. Based on the 16 rRNA, 8 genera of bacteria with a total of 15 species were obtained, they were Aeromonas caviae, Aeromonas salmonicida, Bacillus anthracis, Bacillus cereus, Bacillus mycoides, Enterobacter cloacae, Enterobacter kobei, Klebsiella pneumonia, Myroides profundi, Providencia huaxiensis, Pseudomonas balearica, Pseudomonas hydrolytic:a, Pseudomonas khazarica, Shigella flexneri and Shigella soneii. B. cereus were the predominant strains and were obtaind at each locations. Screening of the potential for decolorization of dye waste on solid MSM (Mineral Salt Medium) containing 25%, 50%, 75% and 100% of dye-waste with an incubation period of 4 days obtaind 5 strains of bacteria with a larger colony diameter, including S. flexneri, B. cereus, A. salmonicida, K. pneumonia and E. doae. Base on colony diameter, jive most potential strains were tester for their decolorization ability in liquid MSM media containing 50% dye-waste with and without the addition of 1% glucose during an incubation period of I 5 days. The percentage of decolorization by the five strains showed a higher decolorization ability at the addition of 1% glucose. The highest decolorization ability was shown by strain A. salmonicida with a decolorization percentage value of 36.2% with the addition of 1% glucose and 25.0% without glucose.