Pengaruh Ekstrak Daun Puguntano (Picria fel-terrae (Lour.) Merr.) terhadap Histomorfometri Aorta Jantung Pada Tikus Galur Wistar (Rattus norvegicus) Model Obesitas dengan Benign Prostat Hyperplasia

Abstract

Obesity is a condition in which there is an accumulation of excess fat in the body. The use of puguntano leaf extract has bioactive compounds that can reduce the level of damage to the aorta. This study aims to determine the effect of leaf extract of puguntano (Picria fel-terrae (Lour.) Merr.) on the histomorphometry of the heart of obese rats (Rattus novergicus) with benign prostatic hyperplasia. This study used a completely randomized design (CRD) with 5 groups and 5 replications. The group in this study consisted of a negative control group (without treatment), positive control (obese mice with BPH and testosterone injection), the first treatment (obese mice with BPH and testosterone injection and given standard drug finesteride), the second treatment (obese mice with BPH and injected with testosterone and given puguntano leaf extract 100 mg/KgBW), the third treatment (obese rats with BPH and injected with testosterone and given puguntano leaf extract 200 mg/KgBW). Obese rats were identified by calculating Body Mass Index (BMI). Aortic organ preparations were made by paraffin method and Hematoxylin Eosin (HE) staining. The results showed that the puguntano leaf extract treatment of 200 mg/KgBW had an effect on improving the histomorphometry of the rat heart which resulted in heart weight, heart volume, aortic wall thickness, aortic diameter, respectively, with an average of 0.83; 1.16; 75.76; 97,42 and reduce the percentage of atherosclerosis frequency to 20%.