Peningka Tan Status Antioksidan Endogen Superoxide Dismutase (SOD) dan Catalase (CAT) pada Fibroblas dan Jaringan Koklea Tikus yang Diinduksi Bising Akibat Pemberian Curcuminoid

Abstract

Background: Noise exposure consequently leads to increased levels of radical oxygen species (ROS) in the cochlea, causing cochlear damage and noise-induced hearing loss (NIHL) Treatments enhancing antioxidant defences might protect susceptible individuals from NIHL

Objective. To demonstrate curcuminoid as the safe and effective therapeutic agent in the prevention and treatment of fibroblasts damage within the cochlear supporting tissues and lateral wall following noise exposure through oxidative metabolic pathway

Methods Twenty-four Rattus norvegicus were randomly divided into 4

groups (n = 6) Group 1 control, group 2: noise (+), group 3 noise (+), 50 mg/day curcuminoid (+), group 4. noise (+), 100 mg/day curcuminosd (+). All groups (except group 1) were subjected to 100 dB SPL for 2 hours/day for 14 days Curcuminoid derived from Curcuma longa Linnaeus (turmeric) with curcumin content levels [28.111 01% w/w compared to standard, was administered orally for 14 days All samples were immunohistochemistrically examined for the expressions of superoxide dismutase (SOD) in cochlear fibroblasts and colorimetrically examined for catalase (CAT) levels in cochlear tissues using colorimetric reader All statistical analyses were performed by Statistical Analysis System (SAS)

Results The results obtained showed significant differences for the expressions of SOD (p<0.05) in all groups, except in group 1 compared to group 4. and CAT levels (p<0.05) in all groups except in group 1 compared to group 4 and group 2 compared to group 3 Group 4 showed statistically significant increase (p<0.05) in the expressions of SOD, and CAT levels compared to group 3

Conclusion Curcuminoid is potentially effective in the prevention and treatment of oxidative damage in fibroblasts within the cochlear supporting tissues and lateral wall following noise exposure by modulating the expressions of SOD and CAT levels.

Latar belakang: Paparan bising mengakibatkan terjadinya peningkatan kadar radical oxygen species (ROS) di koklea, menyebabkan kerusakan koklea dan gangguan pendengaran akibat bising (GPAB). Terapi yang meningkatkan pertahanan antioksidan mampu melindungi individu yang rentan terhadap GPAB. Tujuan penelitian: Membuktikan curcuminoid scbagai agen terapeutik yang efektif dan aman dalam mencegah dan mengobati kerusakan fibroblas jaringan-jaringan penyokong dan dinding lateral koklea melalui jalur metabolik oksidatif. Metode penelitian: Sampel yang digunakan 24 ekor Rattus norvegicus secara acak dibagi menjadi 4 kelompok. Kelompok 1: kontrol, kelompok 2: bising ( + ), kelompok 3: bi sing ( +) curcuminoid 50 mg/hari ( + ), kelompok 4: bising ( +) curcuminoid 100 mg/hari ( + ). Seluruh kelompok (kecuali kelompok 1 ), diberi pajanan bising 100 dB SPL selama 2 jam/hari selama 14 hari. Curcuminoid yang diekstrak dari Curcuma longa Linnaeus (kunyit) dengan kadar curcumin [28.1 ±1.0]% b/b dibanding standar diberi secara oral selama 2 minggu. Seluruh sampel diperiksa secara imunohistokimia untuk ekspresi superoxide dismutase (SOD) pada fibroblas koklea dan secara kolorimetri untuk kadar catalase (CAT) pada jaringan koklea menggunakan colorimetric reader. Seluruh analisis statistk dilakukan dengan Statistical Analysis System (SAS). Hasil: Hasil pemeriksaan dipero!eh terdapat perbedaan ekspresi SOD yang bermakna (p<0.05) secara statistik untuk semua kelompok perlakuan, kecuali kelompok 1 dibanding kelompok 4; dan kadar CAT (p<0.05) untuk semua kelompok kecuali kelompok 1 dibanding 4, dan kelompok 2 dibanding 3. Kelompok 4 menunjukkan peningkatan berrnakna (p<0.05) pada ekspresi SOD, dan kadar CAT dibanding kelompok 3. Kesimpulan: Curcuminoid terbukti efektif berpotensi mencegah dan mengatasi kerusakan oksidatif fibroblas jaringan-jaringan penyokong dan dinding lateral koklea akibat papa ran bising melalui · modulasi terhadap ekspresi SOD dan kadar CAT.