Aktivitas Enzim Peroksidase (POD) Lateks dan Analisis Fisiologi Kulit Karet (Hevea brasiliensis Muell. Arg) Klon PB 260 dan RRIM 921 dengan Pemberian Antidepresan pada Musim Gugur Daun

Abstract

Tapping panel dryness (KAS )significantly decreased the yield and accelerated the plant rejuvenation. Then presence of the leaf fall period also could reduce the value of the rubber production every year. Therefore, the analysis of several variables metabolism was important especially on the rubber plant bark which could be used as an early indicator for the healing of the tapping panel dryness. The research aimed to determine the activity of peroxidase (POD) in latex and the bark analysis of rubber plant (Hevea brasiliensis Muell. Arg) clones PB 260 and RRIM 92 applied with antidepressant on the leaf fall period. The research was conducted at the Laboratory of Physiology Sungei Putih Rubber Research Center from March 2016 to June 2016. The correlation analysis, the T testwere used using the program Minitab 16.0 and SPSS 20.0 with factors i.e. : clones (RRIM 921 and PB 260), types of plants (healthy and KAS) and the formula od the stimulants. Parameter observed were thiol, inorganic phosphate (pi), sucrose, protein and activity of peroxidase (POD). The results showed that the range of values Thiol bark is 0.1-0.9 mM, Pi is 4-20 mM sucrose is 24-60 mM, Protein 40- 65 mg / unit and the POD 0.1-1 units / mg protein changed in accordance with the condition of the plant and environmental factors. Positive correlations obtained on thiol parameter and pi (0534), sucrose and protein (0.926), and sucrose with POD (0.257). The negative correlation obtain in thiol and sucrose (0.134), and protein thiol (0.160), thiol and POD (0.615), pi and sucrose (0.473), and protein pi (0.156), protein and protein and POD (0.529).