Uji Diagnostik Tinea Kruris dengan Polymerase Chain Reaction-Restriction Fragment Length Polymorphism

Abstract

Background: Tinea cruris is the second most common dermatophytosis in the world and the most common in Indonesia. The conventional laboratory tests for dermatophye infection are direct microscopic examination with 10% potassium hydroxide (KOH) and fungal culture, but these tests are slow and less specific. Therefore, there is a need of a more rapid and exact diagnostic methods. Molecular technologies have been found such as polymerase chain reaction (PCR) which is a very sensitive and specific test and may be used to diagnose various

microorganisms including pathogenic fungi. Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP) is a PCR method with

the addition of enzyme after amplification, therefore enabling for more specific results. Objective: To determine the diagnostic value of PCR-RFLP in the diagnosis of tinea cruris, Methods: This study is a diagnostic test tinea cruris vvith PCR-RILP by using culture as the gold standart. Subjects; Thirty-one patients suspected of having tinea cruris from history and dermatological examination. Results: The values of the diagnostic test yielded in this study are the sensitivity value was 75%, the specificity was 66.7%, the positive predictive value was 70,6%, the negative predictive value was 71,4% the positive likelihood ratio was 2.25,the negative likelihood ratio was 0.38, the accuracy value was 70.9%. Conclusions: PCR-RFLP can be used as an alternative tool for the diagnosis of tinea cruris.