| dc.description.abstract | Background: Tinea cruris is the second most common dermatophytosis in the
world and the most common in Indonesia. The conventional laboratory tests for
dermatophye infection are direct microscopic examination with 10% potassium
hydroxide (KOH) and fungal culture, but these tests are slow and less specific.
Therefore, there is a need of a more rapid and exact diagnostic methods.
Molecular technologies have been found such as polymerase chain reaction (PCR)
which is a very sensitive and specific test and may be used to diagnose various
microorganisms including pathogenic fungi. Polymerase Chain Reaction-
Restriction Fragment Length Polymorphism (PCR-RFLP) is a PCR method with
the addition of enzyme after amplification, therefore enabling for more specific
results.
Objective: To determine the diagnostic value of PCR-RFLP in the diagnosis of
tinea cruris,
Methods: This study is a diagnostic test tinea cruris vvith PCR-RILP by using
culture as the gold standart.
Subjects; Thirty-one patients suspected of having tinea cruris from history and
dermatological examination.
Results: The values of the diagnostic test yielded in this study are the sensitivity
value was 75%, the specificity was 66.7%, the positive predictive value was
70,6%, the negative predictive value was 71,4% the positive likelihood ratio was
2.25,the negative likelihood ratio was 0.38, the accuracy value was 70.9%.
Conclusions: PCR-RFLP can be used as an alternative tool for the diagnosis of
tinea cruris. | en_US |
