Uji Sitotoksik Senyawa Aktif Kalkon Tersubstitusi Halogen terhadap Sel Kanker Payudara MCF-7

Abstract

The synthesis of halogen-substituted chalcone compounds was carried out using the Clasien-Schmidt condensation type aldol condensation method, using microwave radiation or ecofriendly. The number of substituted chalcone compounds obtained based on the UV, IR, 1H-NMR and MS structural elucidation were 7 compounds. The seven compounds were (E)-1-(4-chlorophenyl)-3-p-tolylprop-2-en-1-on (1), (E)-1-(4-chlorophenyl)-3-(4-isopropylphenyl)-prop-2-en-1-on (2), (E)-1-(3-bromophenyl)-3-p-tolilprop-2-en-1-on (3), (E)-1-(3-bromophenyl)-3-(4-isopropylphenyl)-prop-2-en-1-on (4), (E)-3-(4-chlorophenyl)-1-o-tolylprop-2-en-1-on (5), (E)-1,3-bis (3-bromophenyl)-prop-2-en-1-on (6) and (E)-3-(4-bromophenyl)-1-(3-chlorophenyl)-prop-2-en-1-on (7) and the yields were 81,03% (highest yield), 64,80%, 62,32%, 55,32%, 60,00%, 60,51% and 74,38%. Cytotoxic bioactivity against cancer cell death of MCF-7 based on IC50 value were carried out by adding PrestoblueTM reagent method and measuring absorbance. Cytotoxic bioactivity based on IC50 values of compounds 1-7 respectively were 1,484.75 g/mL; 37.24 g/mL; 42.22 g/mL; 22.41 g/mL; 3.66 g/mL; 51.05 g/mL and 21.62 g/mL. The highest cytotoxic bioactivity was compound (E)-3-(4-chlorophenyl)-1-o-tolylprop-2-en-1-on (5) at 3.66 g/mL.