Pengaruh Nanoherbal Daun Biwa (Eriobotrya japonica (Thunb.) Lindl.) terhadap Aktivitas Antiinflamasi Pada Tikus (Rattus norvegicus) yang diinduksi Karagenan

Abstract

Inflammation is a body’s protective response to injury or tissue injury caused by the presence of pathogenic agents and foreign objects, physical trauma, or harmful chemicals. Loquat leaf nanoherbs are used because of the presence of compounds that have anti-inflammatory activity. The purpose of this study was to determine the effect of giving loquat leaf nanoherbs on anti-inflammatory activity and leukocyte profile in carrageenan-induced rats. This study used a sample of 25 healthy male white rats weighing 150-250 gr, which were divided into 5 groups. The positive control group was the Na-diclofenac suspension, the negative control group was the 0.5% Na-CMC suspension and the loquat leaf nanoherbal treatment group was 125, 150, and 175 mg/kgBW body weight. The anti-inflammatory activity test was carried out using the paw edema method using a digital pletismometer to see the ability of the loquat leaf nanoherbal in inhibiting the volume of inflammatory swellings formed and blood tests were carried out at the Regional Health Laboratory of North Sumatra Province. The results showed that the administration of loquat leaf nanoherbs at doses of 125, 150, and 175 mg/kgBW had significantly different antiinflammatory activity with the negative control group, but not significantly different with the positive control group. The Nanoherbs with a dose of 175 mg/kgBW gave a better anti-inflammatory effect because it was close to the positive control value at 6 hours with inflammation percentage of 11.19% and inflammation inhibition percentage of 77.34%. Loquat leaf nanoherbs can reduce the total number of leukocytes and can affect the type of leukocyte count in rats, especially neutrophils and lymphocytes, but does not affect at the number of other types leukocytes, namely monocytes, eosinophils and basophils