Pengaruh Vitamin E terhadap Pemulihan Spermatozoa Mencit (Mus musculus L.) yang Mendapat Ekstrak Air Biji Pepaya (Carica papaya L.) dan Testosteron Undekanoat (TU)

Abstract

The combination of water extracted of papaya seed and Testosterone Undecanoate (TU) may induce reducing quantity and quality of spermatozoa. The compound in extracted of papaya seed may generate oxidative stress as its indication of existing free radical. Vitamin E is capable to scavenger free radical in its function as antioxidant, it neutralize free radical and inhabiting lipid peroxide. The research on the effect of vitamin E in recovering of spermatozoa mice (Mus musculus L.) with its water extracted of papaya seed (Carica papaya L.) and TU was done aiming to determine the effect of presenting vitamin E for recovering mice of spermatozoa, following given extract of papaya seed and TU. The subject of study are ripen mice spermatozoa (Mus musculus L.) of adult fertile (3 months old) with body weight 25-35 grams, about 70 pieces classified into 7 groups in treatment of each comprising control 35 mice. The mice of group comprising control 35 pieces and treatment 35 pieces of mice. The long of presenting extracted papaya seed was 24 weeks that presented every day and TU with interval 6 weeks starting week 0 (P0) through 24 (P4). Then continued with presenting vitamin E for mice of week 24th (P4), 30 (P5) and 36 (P6) about 8iu/0,2 ml/ mice orally. Examinations of quantity and quality of spermatozoa was conducted by using improved Neubaeur and in examination of spermatozoa morfologi using giemsa coloring. The data was analyzed by using SPSS release 13, non parametric later continued with T test. The result of test indicated that any treatment by presenting vitamin E on mice (Mus musculus L.) that has got combination of water extracted papaya seed and TU capable to maintain and recorver its quantity, motility, viability and morphology of spermatozoa, so concluded the vitamin E is capable to recorver its quantity and quality of spermatozoa.