Uji Kemampuan Bakteri Kitinolitik dari Nepenthes Tobaica dan Nepenthes Gracilis dalam Menghambat Pertumbuhan Beberapa Jamur Patogen Tanaman

Abstract

The ability of chitinolytic bacteria isolated from Nepenthes tobaica and Nepenthes gracilis to inhibit the growth of plant pathogen fungi has been studied through antagonistic and crude extract tests. Five chitinolytic bacterial isolates isolated from Nepenthes tobaica and Nepenthes gracilis have been tested and showed the ability to inhibit the growth of Fusarium sp. and Rhizoctonia solani. RH1 isolate has the highest ability to inhibit the growth of Fusarium sp. and Rhizoctonia solani with diameter of inhibition zone of 2.00 cm and 2.40 cm, respectively. AM1 isolate showed relatively the same diameter of inhibition of 2.05 cm and 2.15 cm, respectively. CBH isolate showed the lowest inhibition zone of 0.90 cm and 1.60 cm respectively. The crude extract of the chitinase of isolates with the highest inhibitory ability was tested back against of both fungi. The crude extract of RH1 showed inhibition of the growth of Fusarium sp. and Rhizoctonia solani with inhibition zone of 0.91 cm and 1.41 cm, respectively while extract of AM1 showed inhibition zone of 1.35 cm and 1.27 cm, respectively.