Isolasi dan Uji Kemampuan Bakteri Endofit Penghasil Hormon Iaa (Indole Acetic Acid) dari Akar Tanaman Jagung (Zea Mays L.)

Abstract

Research on Isolation and Ability Test Of Endophytic Bacteria Producing IAA (Indole Acetic Acid) Hormone From Crops Maize Root (Zea mays L.) has been carried out from September 2008 to September 2009 at the Laboratory of Microbiology Faculty of Mathematic and Natural Science and Laboratory of Quantitative Chemistry Faculty of Pharmacy North Sumatra University . This study was aimed to obtain isolate of endhophytic bacteria producing IAA of corn plant roots and to know their role in promoting seed germination of corn. Bacteria producing IAA was isolated, characteristed, and tested their ability to produced IAA both in vitro or in vivo. The results showed that there were 13 potential endhophytic bacteria isolates that produced IAA. The highest IAA concentration was 1.1255 ppm obtained from isolate KB3. Soaking seedling into bacterial suspensions could potentially enhanced the growth of seedling. Parameters observed were sprout height, root length and sprout weight.