Analisis Keanekaragaman Genetik Markisa (Passiflora spp.) di Sumatera Utara berdasarkan Penanda RAPD (Random Amplified Polymorphic DNA)

Abstract

The research about Genetic diversity analysis of the passion fruit (Passiflora spp.) in north Sumatera based on RAPD markers has been done in Genetic Laboratory and Integrated Laboratory, University of North Sumatera. The objective of this study was to characterize the genetic diversity of Passiflora sp. collection by RAPD marker. RAPD analysis was done to 31 passion fruit (Passiflora sp.) accessions that collected from 4 regional is region HUMBAHAS, North Tapanuli, Simalungun, and Karo that represented 4 species (P. edulis, P. ligularis, P. quadrangularis, P. foetida) from genus of Passiflora using 4 random primers (selected from 7 random primers). The result of electrophoresis showed 73 reproducible polymorphic bands (98%) are ranging in size from 250 bp to 2500 bp. The genetic similarity showed coefficients ranged from 0,8 to 0,97 based on Unweight Pair- Group Method Aritchmatic and Numerical Taxonomy (UPGMA) and Multivariate System (NTSYS) version 2.11a computer program,. The result revealed high levels of variation within and among species. Cluster analysis showed that grouping of passion fruit occurs by area collecting of the sample. Geographical proximity and similarity of the area where passion fruit had been collecting and based on the color of the fruit do not guarantee the genetic relationship of passion fruit by RAPD.