| dc.description.abstract | The research about Genetic diversity analysis of the passion fruit (Passiflora spp.)
in north Sumatera based on RAPD markers has been done in Genetic Laboratory
and Integrated Laboratory, University of North Sumatera. The objective of this
study was to characterize the genetic diversity of Passiflora sp. collection by
RAPD marker. RAPD analysis was done to 31 passion fruit (Passiflora sp.)
accessions that collected from 4 regional is region HUMBAHAS, North Tapanuli, Simalungun, and Karo that represented 4 species (P. edulis, P. ligularis, P.
quadrangularis, P. foetida) from genus of Passiflora using 4 random primers
(selected from 7 random primers). The result of electrophoresis showed 73
reproducible polymorphic bands (98%) are ranging in size from 250 bp to 2500
bp. The genetic similarity showed coefficients ranged from 0,8 to 0,97 based on
Unweight Pair- Group Method Aritchmatic and Numerical Taxonomy (UPGMA)
and Multivariate System (NTSYS) version 2.11a computer program,. The result
revealed high levels of variation within and among species. Cluster analysis
showed that grouping of passion fruit occurs by area collecting of the sample.
Geographical proximity and similarity of the area where passion fruit had been
collecting and based on the color of the fruit do not guarantee the genetic
relationship of passion fruit by RAPD. | en_US |
