Isolasi dan Identifikasi Bakteri Kitinolitik dari Nepenthes tobaica dan Nepenthes gracilis

Abstract

Nepenthes, a pitcher plant obtains its nitrogen from degrading trapped insect in the pitcher. Chitinolytic bacteria are assumed acting in degradation of exoskeletons of insects in the pitcher. In this study, chitinolytic bacteria were isolated from the fluid of opened and closed pitchers, pitchers, stalks, roots, and rhizosphere soil of Nepenthes tobaica and Nepenthes gracilis from Merek, Dairi, North Sumatra. Total of 18 isolates were obtained by isolating the bacteria on minimum salt media chitin adding with 0.2% yeast extract. Two isolates that have higher chitinolytic index, AM1 was from the root of N. tobaica with chitinolytic index of 2.25, and CbM was from the fluid of opened pitcher of N. tobaica with chitinolytic index of 1.90. Identification of 16S rRNA gene indicated that CbM isolate was closely related to Enterobacter aerogenes strain VT66, while AM1 was not succeeded to be identified.