Pengaruh Pemberian Ekstrak Segar Buah Andaliman (Zanthoxylum acanthopodium DC.) terhadap Gambaran Histologis Ginjal Mencit Jantan (Mus musculus L.)

Abstract

The Histologic Description of Male Mice (Mus musculus L.) Kidney Treated With Fresh Andaliman (Zanthoxylum acanthopodium DC.) Fruit Extract has been studied from March 2013 to November 2014. The study used completely randomized design (CRD) consisting of 4 treatment groups; control (K0), and treatment with 2% (P1), 5% (P2), and 10% (P3) of fresh andaliman fruit extract for 40 days and continued for 40 days without treatment to examine recovery. Each treatment group consisted of 6 mice. Mice were dissected after 40 and 80 days of treatment, the kidneys were obtained, weighed, and made into microscopic slides for histologic examination using paraffin method and Hematoxyline Eosin (HE) stain. Morphological examination showed color change in P2 treatment group. No significant difference was observed in kidneys weight between treatment and control group. Histologic examination of glomeruli diameter and proximal tubuli diameter showed an increase in diameter of glomeruli in all treatment groups (P1, P2, and P3) but a decrease in diameter of proximal tubuli in P1 treatment group compared to control group. Recovery phase showed a decrease in proximal tubuli diameter compared to pre-recovery phase.