Hubungan Ekspresi p16 dengan Morfologi Inti Koilosit pada Lesi Kondiloma Akuminata

Abstract

Background: Condyloma Acuminata or genital warts is a Sexually Trarxmitted Infections disease caused by the Human Papilloma Virus (HPV). Research has done to determine the effect of HPV against tumor suppressor protein, HPV oncogenic associated with viral proteins E6 and E7 which leads to increased cell proliferation resulting in pre-cancerous lesions that can develop into cancer. Pl6 is a tumor suppressor gene that produces the protein p16 which serves as a binder CDK4 / CDK6 which inhibits the cell cycle. Detection of proteins that play a role in the regulation of the cell is expected to be used as a biomarker which enables early detection of cancer. Koilocytes is a pathognomonic sign of HPV infection The formation of cytoplasmic vacuoles is unclear, but mainly due to HPV DNA replication and virion contained in nucleus. Objectives: To access correlation between expression ofpl6 and nuclear morphology

of koilocytes on condyloma acuminata lesions- Subject and methods: The subjects of this study included 31 samples with

condyloma acuminata lesions, history taking physical, dermatovenereological, acetowhite examination, biopsy of the lesion, and immunohystochemical staining were done on all the subjects. This was an analitic study with cross-sectional design. Results: There was no significant correlation between the expression of p16 with nuclear morphology of koilocytes on condyloma acuminata lesion.(p{,5). Twenty one lesions of condyloma acuminata drow positive/p 16 overexprcssion, 20 lesio'ns showed nuclear morphology of koilocytes B in normal size or small, round to oval shapes, regular edge, staining dense, homogeneous, piknotic