Deteksi Protein C-myc pada Jaringan Uterus Mencit (Mus musculus L.) dengan Metode Imunohistokimia

Abstract

Cancer is a kind of disease caused by many factors, one of them is cell cycle protein mutation namely c-myc protein. C-myc protein is the master regulator of cell metabolism and proliferation. The c-myc protein is generally linked with cancer. This research aims to detect the c-myc protein on the tissue. the tissues are selected based on davies’ theory, namely the lifespan of younger tissue are more susceptible to cancer and vice versa, that’s why uterine tissue of mice (Mus musculus L) are chosen in which its evolution lifespan is more easily compared than ovarium tissue of mice (Mus musculus L.) The method applied is immunohistochemistry namely the antibody antigen binding reacted with immunohistochemistry kit to cause the brown color on the tissue expressed c-myc protein. The obtained result showed that c-myc protein in the uterine tissue is expressed in lamina propria (stroma) between the gland and the level of expression is higher with a score of 2.5 is a medium expression, whereas on the ovary, c-myc protein is expressed in theca cells, granulosa cells and oocytes with expression levels 1.75 which is low expression.