Potensi Bakteri Filosfer Tanaman Ornamental untuk Menghambat Pertumbuhan Mikroorganisme Bioaerosol Berpotensi Patogen dari Lingkungan Rumah Sakit Umum Pirngadi Medan

Abstract

Uncontroled bioaerosol in a hospital environment can be the source of nosocomial infection. An alternative way to overcome the transmission of this infection is needed. This study aimed to determine the potention of phyllosphere bacteria from ornamental plants Ficus elastica, Philodendron bipinnatifidum and Aglonema simplex in controlling the growth of potentially pathogenic bioaerosol microorganism. Bioaerosol bacteria isolated from ambient air of RSU dr. Pirngadi, Medan using MSA, MC, PCA and SDA plate exposed to the air for 15 minutes. The most dominant isolated bioaerosol bacteria were Staphylococcus aureus, Staphylococcus sp., Bacillus sp., Streptococcus sp., Pseudomonas sp., Salmonella sp., Shigella sp., and fungi of the genus Aspergillus, Alternaria, Trichoderma, Neurospora, Fusarium and Penicillium. Inhibitory activity test results indicated that phyllosphere bacteria of A. simplex had the highest inhibitory effect to the growth of bioaerosol fungal. The most potential isolate was AS2 with inhibition zone against Mucor sp. was 4.2 cm. Phyllospheric bacterial inhibition of P. bipinnatifidium isolate PB2 was 3.7 cm against Mucor sp.,while the highest inhibition of phyllospheric bacteria of F. elastica showed by isolate FE2 was 2.4 cm against Mucor sp. The identification result of three phyllosphere bacteria belong to Pseudomonas spp. This study concluded that phyllospheric bacteria from ornamental plant had better inhibition for against bioaerosol fungal rather than bioaerosol bacterial.