Pengaruh Penambahan Amonium Sulfat dan Sukrosa pada Media Air Kelapa terhadap Produksi dan Karakteristik Fisik Selulosa Mikrobial Acetobacter xylinum

Abstract

Acetobacter xylinum is a bacterium that produces microbial cellulose which has been commonly used in several industries factory such as food industry, paper, electronics, pharmacy, and biomedical. Study about the effects of ammonium sulphate and sucrose addition into coconut water media on the production and physical characteristics of microbial cellulose by Acetobacter xylinum has been conducted. Ammonium sulphate were 0, 0.5, 1 and 1.5 % which were combined with 0, 2, 4, 6 % of sucrose. The cultures were growth for three days. Microbial cellulose of each treatments was analysed based on the physical properties such as rendemen, thickness, water content and tensile strength . The higher sucrose concentration added to the medium, the better production and physical characteristics of microbial cellulose. Concentration of ammonium sulphate 1 % was produce the best quality of microbial cellulose, while 1.5 % decreased the production and the quality of microbial cellulose. The highest rendemen, thickness, water content and the tensile strength of microbial cellulose was 31.23 %, 8.77 mm, 95.54 %, 3.234 MPa, respectively. The combination treatment of 1 % ammonium sulphate and 6 % of sucrose produced the best value of rendemen, thickness, water content and the tensile strength. However statistical test showed that there were no significant different among the treatment.