Isolasi dan Uji Kemampuan Antifungal Bakteri Endofit dari Andaliman (Zanthozylum Acanthopodium Dc.) terhadap Fungi Perusak Makanan

Abstract

The Isolation and Ability of Antifungal Endophitic Bacteria from Andaliman (Zanthoxylum acanthopodium DC.) to inhibit The growth of Food Spoilage Fungi had conducted since January to October 2009 in Microbiology Laboaratory, Mathematic And Natural Sciences Faculty, Sumatera Utara University, Medan. Endophytic bacteria isolated from root, stem, leaves, and fruit were found from Paung village Dolok Sanggul street, Sidikalang junction 3, Pollung, Humbang Hasundutan County, North Sumatera, then characterized and the ability of the antagonist was tested for Aspergillus sp1 and Aspergillus sp2. The fungi were isolated from decomposed rice and cake. The result found that 30 isolates of bacteria from 12 kinds from fruit and 6 kinds each from root, stem, and leaves. The result of Gram staining show that 30 isolates as much as 46.66 are Gram + bacteria and 53.33 % are Gram - bacteria. Most of the bacteria are potential to inhibit the growth of mycelium of Aspergillus sp1 or Aspegillus sp2, meanwhile 12 isolates are not potential. The isolate that has a great potential in AD03 marked with a great inhibitory zone that resulted and the mycelium are dry, branches, and thin.