Perubahan Fenotipe Ulat Sutera (Bombyx Mori L.) yang Diinduksi dengan Sinar Ultraviolet (Uv) dan Kariotipe Kromosom

Abstract

Research on "Change Phenotype Silkworm (Bombyx mori L.) is Induced by Ultraviolet (UV) and Chromosome Karyotype" has been done in July to December 2009 at the Laboratory of Genetics, Department of Biology, University of North Sumatra, Medan. This study aims to determine changes in the silkworm phenotype induced by ultraviolet (UV) and to know the silkworm karyotype. This research uses Completely Randomized Design (CRD) with 30 Watt and intensity exposures 0; 1.5, 3.0 and 4.5 minutes. The results of the average data length of morphology indicates that the increase in long irradiation with UV light energy causes a decrease in 30 Watt long-VI instar body size, instar V, wings, body and anther in silk moths. But from a statistical analysis shows that the increase in irradiation time to give real effect on body length IV instar larvae and silk moth body length, body length, while the V instar larvae, long wings and long antennae that do not show effects significantly different. Providing 30 watts of UV light causes a change in phenotype in the form of wing-wing type which appears as mutant vestigial wing, minute wing and wrinkled wing. And change the body color of larva instar V Inhibitor-f Lemon and dilute black. Karyotype Bombyx mori L. Has a number of 56 pieces or 28 pairs (2n) consisting of 3 types of chromosomes with chromosome type metacentric, submetacentric and telocentric. The longest chromosome is a chromosome length of chromosome 1 with a 1.14 μm with metacentric chromosome types whereas the shortest chromosome is chromosome number 28 with a length of 0.15 μm chromosome chromosome type telocentric. Percentage of relative arm length (% PR) is the largest chromosome 1 with chromosome length percentage of 9.41% and the relative length of chromosome arm is the smallest chromosome number 28 with a value of 1.18% and the percentage of centromere index (% IS) which is a chromosome spread 27 and 28 with a value of 100%, and% IS smallest is chromosome number 20 with nilai17, 85%. Universitas