Isolasi dan Uji Kemampuan Bakteri Endofit Penghasil Hormon Iaa (Indole Acetic Acid) dari Akar Tanaman Padi (Oryza Sativa L.)

Abstract

Research on Isolation and Ability Test of Endophytic Bacteria Producing IAA (Indole Acetic Acid) Hormone from Paddy Roots (Oryza sativa L.) has been carried out in the Laboratory of Microbiology, Faculty of Mathematic and Natural Science and Laboratory Quantitative Chemistry, Faculty of Pharmacy, University of Sumatera Utara. The objective of this research was to isolate endophytic bacteria producing IAA hormone from paddy roots and their role in promoting germinated paddy. The isolates were morphological and biochemically characterized and the ability test of producing IAA was analyzed by colorimetric. The highest IAA concentration was obtained by the isolate of Bj2 which 1,090 ppm. Meanwhile in vivo test was done using sprout soaked with bacterial suspension. Soaking sprouts into bacterial suspension could potentially enhance of growth of paddy sprouts. Isolate Md 1 has showed best result with sprout height was 25,58 cm; root length 10,48 cm and sprout weight 0,2 g.