Isolasi Dan Uji Antagonis Fusarim Sp. pada Tanaman Anggrek Menggunakan Bakteri Kitinolitik Lokal

Abstract

An Assay On Isolation And The Antagonistic Test Of Fusarium sp. Isolated From Some Orchid Plants Against Local Chitinolitic Bacteria, has been conducted in Laboratory of Microbiology, Department of Biology, Faculty of Matematics and Natural Sciences, Sumatera Utara University, Medan from May to December 2010. The purpose of the research was to study the ability of bacteria isolated from Bangka, Langkat and Deli Serdang (from previous study) to inhibit the growth of Fusarium sp 1., isolased from Cattelya sp; Fusarium sp. 2 from Vanda sp.; Fusarium sp. 3, Fusarium sp. 4, and Fusarium sp. 5, from Phalaenopsis sp on isolation from orchid plantation Tiga Dolok, District Simalungun, Sub-District Dolok Panribuan, and the existence of fungal pathogens by noting the simptom. Isolation of pathogenic fungi was conducted by direct plating method placing 3 cm slices of diseased orchid of roots, leaves and stems on sterile PDA medium containing chloramphenicol and yeast extract. The isolates then were incubated at room temperature for 72 hours. Colonies were cultured on sterile Petri dishes to ensure the existence of fungal pathogens. The obtained fungi were isolated and identified using identifiction book of fungi. Based on the antaonistic test, it was shown that the most effective bacteria in inhibiting the growth of Fusarium sp. 5, was BK15, with inhibition zone radius of 2.82 cm. Whereas, the least effective bacteria was BK13, with inhibition zone radius of 0,42 cm.. And for abnormal hyphae Fusarium sp. 1, Fusarium sp. 2, Fusarium sp. 3, Fusarium sp. 4, dan Fusarium sp. 5, in experienced lysis, rolled and twisted after the test antagonistic.