| dc.description.abstract | Salmonella sp., Staphylococcus aureus, and Escherechia coli are opportunistic
pathogenic bacteria found in shrimp ponds and able to form biofilm on solid
surfaces. The purpose of this study was to determine the ability of antimicrobial
compounds produced by Bacillus sp. in inhibiting the growth of opportunistic
pathogenic bacterial biofilms. Opportunistic pathogens bacteria were isolated
from water, sediment and from solid surface using cotton swab of intensive
shrimp ponds in several location in North Sumatera Province. Opportunistic
pathogenic bacteria were antagonist tested against isolates of Bacillus sp. B1, B4,
B9, B11, and B12, to see the ability of inhibition of Bacillus sp. Isolate of
Bacillus sp. B9 were able to inhibit 3 types of pathogen bacteria, while B12 only
able to inhibit two types of pathogenic bacteria, except Escherechia coli.
Opportunistic pathogenic bacterial biofilm was form using stainless steel as an
attachment surfaces, incubated for 1, 3, and 6 days, then immersed in 10 ml of
antimicrobial crude extract and the number of bacteria viability were counted.
Biofilm cells increased with time of incubation, except for Escherechia coli that
decreased on the 6th day of incubation. The crude extract of antimicrobial
compounds of Bacillus sp. B9 and B12 isolates were able to reduce the
opportunistic pathogen bacteria biofilm up to 102
-103 CFU/ml within 24 hours. | en_US |
