Aktivitas Enzim Peroksidase dan Polifenol Oksidase pada Kalus Terung Belanda (Solanum Betaceum Cav.) Setelah Diinduksi Kolkisin

Abstract

The study on “Peroxidase and Polyphenol Oxidase of Tamarillo (Solanum betaceum Cav.) Calli Induction by Colchisine has been done on April to August 2009 in Tissue Culture Laboratory, Biology Departement of North Sumatera, Biomoleculer Laboratory of PPKS Marihat, Pematang Siantar, Quantitative Pharmacy, Pharmacy of North Sumatera and Material of Chemical, Agriculture of North Sumatera. The objective of the research was to obtain the effect of the best concentration and submertion time in colchisine to activity of peroxidase and polyphenol oxidase of tamarillo calli. The research used Completly Randomized Design with 2 factors, which is 4 levels of colchisine (0; 0,01; 0,1 and 1%) and 3 levels submertion time (5, 15 and 30 minutes). The statistical analysis showed that with concentration affected to fresh weight of calli and the enzyme activities, however, the submertion time is fluctuation. The calli treated 0,1% colchisine has the great value of fresh weight 1,32 g and the lowest of fresh weight 1% (C4) with the value 1,14 g. The colchisine treatment of C3T3 indicated the great value of PO activity was equal to 0,0082 unit and meanwhile the PPO activity was 0,0066 unit for the treatment of C2T3. The lowest value of peroxidase activity was 0,0068 unit in C4T1, and PPO activity was 0,0041 unit in C1T3.