Studi Testosteron Plasma, Kuantitas dan Kualitas Spermatozoa Mencit (Mus musculus L.) setelah Pemberian Kombinasi Hormon Testosteron Undekanoat (TU) dan Ekstrak Air Biji Blustru (Luffa aegyptica Roxb.)

Abstract

The aim of this study was to determine the influence of a combination of TU and blustru seed extract against plasma testosterone, the quantity (number of spermatozoa), and quality (motility, viability and morphology) of mice sperm. The study was conducted with complete randomized design with five control and five treatment groups with TU injection of 0.25 mg / mice with a time interval of TU 6 weeks, each time giving a combination of 0, 6; 12; 18; 24 weeks and the provision of seed extract blustru 270mg/mice/day. Each combination of a duration, mice were dissected and taken cauda epididymis and blood of mice. Blood was analyzed to get the data content of plasma testosterone whereas cauda epididymis were observed to obtain data on the quantity and quality of spermatozoa of mice. The data were analyzed with SPSS 15.0 for Windows. The results showed that at duration of 18 weeks of treatment caused significantly declining of plasma testosterone content and both quantity and quality of spermatozoa at low and has a significant difference (p <0.01) between control and treatment. At 24 week was a period for recovery of plasma testosterone contents and quality of spermatozoa but quantity of spermatozoa of mice didn’t under go recovery period because decreased number of mice spermatozoa. From the results of this study, it was concluded that administration of the combination of testosterone undecanoate and blustru seed extract could lower sperm quality and quantity, plasma testosterone, and showed a recovery period except for the quantity of spermatozoa.