Potensi Isolat Bakteri Asal Laut Sibolga dan Tanjung Balai dalam Mendegradasi Minyak Solar

Abstract

The potency of bacteria isolated from Sibolga and Tanjung Balai seashore in degrading diesel oil has been studied in the Microbiology Laboratory Departemen of Biology, Faculty of Mathematics and Natural Sciences University of Sumatera Utara from May to December 2010. Three isolates were used: TJB 01, TJB 05 and SBG 05 . The ability of bacterial isolates in degrading diesel oil was determined based on the growth and decrease the concentration of the composition of diesel oil such as toluene, undecane, ethylbenzene and nonane. The observation of bacterial growth was done by SPC methods on day 0, 4, 8 and 12. The reduced concentration was analyzed using GC, performed on days 0, 4, 8 and 12. Among 3 isolates of bacteria showed that SBG 05 showed the better growth at day 4 (323x10 12CFU/ml), at day 8 (701x1012CFU/ml) and at day 12 (278x1012CFU/ml). The growth of bacterial isolates is line with their ability to degrade diesel oil. Isolat SBG 05 representing most isolat have potency to in diesel oil degrade, posed at from its ability degrade diesel oil component which consist of ethylbenzene 80,23 ppm (70,46%) at day 4 and 76,4 ppm (67,10%) at day 12. Isolat TJB 01 can degrade nonane equal to 85,66 ppm ( 65,54%) at day 4. Then, result shows that the growth of bacterial isolates is line with their abitity to degrade diesel oil.