Penghambatan Serangan Sclerotium rolfsii Penyebab Rebah Kecambah pada Kedelai dengan Bakteri Kitinolitik

Abstract

A study about the inhibition of Sclerotium rolfsii causal of soybean damping off with chitinolytic bacteria had been done in Laboratory of Observation Pest and Disease UPT-BPTPH 1 and Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Medan. The study was aimed to know the ability of chitinolytic bacteria to inhibit S. rolfsii. Six isolates of chitinolytic bacteria showed different ability in inhibiting of S. rolfsii. The most effective isolate was shown by Bacillus sp. BK13 and Enterobacter sp. BK15 with inhibition zone of 3.75 cm and 3.70 cm, respectively. The lowest effective was shown by Enterobacter sp. PB17 with inhibition zone of 0.10 cm. Enterobacter cloacae LK08 do not have ability to inhibit S. rolfsii. BK13 and BK15 isolates shown to reduce damping off on soybean. The seeds soaked in bacterial suspension of BK13 dan BK15 was able to reduce damping off by 44.4% and 50%, respectively.