Kemampuan Bakteri Tanah dalam Menghambat Pertumbuhan Ganoderma Boninense dan Fusarium Oxysporum Secara In Vitro dan Uji Penghambatan Penyakit Layu Fusarium pada Benih Cabai Merah

Abstract

A study on the in vitro ability of soil bacteria to inhibit the growth of Fusarium oxysporum and Ganoderma boninense and inhibition assay of Fusarium wilt disease of chili seeds has been done in the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Medan, started from April 2011 to July 2011. Twelve bacterial isolates showed ability to inhibit the growth of F. oxysporum and G. boninense to some extents. The most effective chitinolytic bacteria and antifungal bacteria to inhibit the growth of F. oxysporum was BK15 and KM01 with diameter of inhibition zone by 20.45 mm and 10.2 mm, respectively. Whereas, BK14 and KM04 showed relatively smaller diameter of inhibition by 3.98 mm and 6.69 mm, respectively. The growth of G. boninense was inhibited most effectively by BK17 and KM04 with diameter of inhibition of 22.74 mm and 14.19 mm, while the less effectiveness of the inhibition was showed by BK14 and AW02 with diameter of inhibition of 11.08 mm and 4.6 mm respectively. Chitinolytic bacterial isolates and antifungal bacterial isolates used to cover red pepper seed through soaking enabled to reduce seeds dumping off. The combination of BK13 and KM04 had the highest inhibition to reduce the dumping off by 83,33% and isolates KM04 (33,33%) had the lowest ability.