| dc.description.abstract | A study of the effect of water salinity to pathogenicity of viral nervous necrosis in
seed tigers grouper (Epinephelus fuscoguttatus) was conducted from January 2011 to
April 2011 at the Laboratory of Molecular Biology of Fish Quarantine Center Class I
Polonia, Medan and the Laboratory of Anatomy Pathology and Laboratory of
Biochemical Investigation Center and Veterinary Testing Regional I Medan. The
study aim was to determine the effect of salinity to the pathogenicity of viral nervous
necrosis (VNN) in tiger grouper infected with VNN. Completely Randomized Design
(CRD) was used in this study. The study consisted of five salinity treatments namely
S20 (20 ppt), S25 (25 ppt), S30 (30 ppt), S35 (35 ppt) and S40 (40 ppt) and control
K30 (30 ppt) without infection of VNN. Each treatment with three replication of 10
fish/20L sea/aquarium. Fish was injected with the virus intra muscular of 10-4/0,1ml
(FID50). The data were analyzed using SPSS release 13.
The results showed that salinity level affected pathogenicity of VNN, (p <5%).
S30 showed high pathogenicity of VNN to the fish while S25 showed low
pathogenicity. Some confirmation tests like hematology tests showed that erythrocytes
number decreased while leukocytes number increased. Histopathology test showed
that there were vacuolization around glial cells and the obstruction of blood vessels
containing erythrocytes. RT-PCR test confirmed that S30 showed highest level of
pathogenicity, while the S25 showed the lowest one. Of all the test confirmation
showed the effect of salinity on the pathogenicity level of VNN in tiger grouper seed. | en_US |
