Isolasi dan Uji Ekstrak Metanol Bakteri Endofit Tapak Dara (Catharanthus Roseus) dalam Menghambat Pertumbuhan Beberapa Mikroba Patogen

Abstract

A study of isolation of endophytic bacteria from tapak dara (Catharanthus roseus) and examination of methanol extracts in inhibited the growth of several pathogenic microbes such as Aspergillus flavus, Streptococcus mutans, Salmonella typhii and Escherichia coli has been conducted. Examination of methanol extract of endophytic bacteria was done with concentrations of 40, 60, 80, and 100%. Methanol extract was prepared by maceration method and centrifugated at 5000 rpm. Examination of isolates and antimicrobial activity of methanol extract of endophytic bacteria was carried out by the disc diffusion method. Eight out of twelve endophytic bacteria isolates were able to inhibit all tested pathogenic microbes wich were BF1, BF2, AFN7, AFN8, AFN9, AF2, AF3, and AF4. Based on diameter of inhibition zone BF1 and AFN9 isolates were found as the most potential isolates in control tested pathogenic microbes. Methanol extract of BF1 and AFN9 isolates was able to inhibit all tested pathogenic microbes. Both of this isolates more inhibit E. coli compare to other pathogens at concentration of 100% .