Pengaruh Pemberian Vitamin C dan E terhadap Gambaran Histologis Testis Mencit (Mus musculus L.) yang Dipajankan Monosodium Glutamat (MSG)

Abstract

This study aimed to determine the effect of vitamin C and E on testicular histology of mouse following exposure to monosodium glutamate (MSG) for 30 days. Experimental used Completely Randomized Design (CRD) consisting of 6 treatments and 5 replications. Mice were treated daily by oral gavage with 4 mg MSG (P1), 4 mg MSG + 0.26 mg vitamin C (P2), 4 mg MSG + 0.026 vitamin E (P3), and 4 mg MSG + 0.26 mg vitamin C + 0.026 mg vitamin E (P4). All dosis were in mg/g body weight. Two control groups K- and K+ received water and castrol oil respectively. The results showed that MSG decrease testis weight and testis volume (P<0,05), also decrease diameter of seminiferous tubule and the number of spermatogenic cells. Vitamin C recover testis weight (P<0,05), but cannot recover testis volume, diameter of seminiferous tubules and the number spermatogenic cells (P>0,05). Vitamin E increase the weight and volume of the testes and spermatogenic cell number (P<0,05), but unable to recover the diameter of the seminiferous tubules (P>0,05). Combination of vitamin C and E recover testicular weight and volume, seminiferous tubule diameter and the number of spermatogenic cells (P<0,05).