Pengaruh Pemberian 2,4-D dan Frekuensi Subkultur terhadap Perubahan Genetik Kalus dari Bunga Betina Kelapa Sawit (Elaeis guineensis Jacq.)

Abstract

The study of “The Effect of 2,4-D And Subculture Frequencies on Genetic Changes of Callus from Immature Female Inflorescences Of Oil Palm (Elaeis guineensis Jacq.)” has been done from March 2013 to April 2014 at Plant Tissue Culture Laboratory of Faculty Mathematics and Science, University of Sumatera Utara. The experimental design was completely randomized with four levels of 2,4-D concentrations: 0; 325; 400; 475 µM and three subculture frequencies: 3; 4; 5 times. The statistical analysis showed that 475 µM of 2,4-D significantly affected the callus initiation and fresh weights from immature female inflorescences (p < 0,05). The RAPD marker showed that 4 and 5 times subculture caused genetic changes.