Pengaruh Salinitas terhadap Aktivitas Enzim Lipase dari Bacillus cereus DA 5.2.3 dalam Degradasi Pakan Udang

Abstract

One of the serious problems of intensive shrimp of aquaculture is the accumulation of shrimp feed at the bottom of shrimp farms. One content of shrimp feed is lipid. Bacillus cereus DA 5.2.3 was expected to degrade lipids in shrimp feed at different concentrations and salinities. Early detection of lipase was conducted by the addition of fish oil and methyl red. It was then continued by the test of lipase activity which was measured by the levels of free fatty acids by the method titimetri. Lipase activity was detected in medium of fish oil and the highest activity of lipase was found at 0‰ salinity and shrimp feed with a value of 1 g/ 5 ml with the free fatty acid content of 0.25%. However, lipase from Bacillus cereus 5.2.3 DA was active in the range of salinity of shrimp aquaculture with the free fatty acid level around 0.1%.