| dc.description.abstract | Probiotics are living microorganisms that provide health benefits to the host.
Probiotics in the form of free-cells may decrease cell viability against
environmental influences. This study aimed to get the lactic acid bacteria as
probiotic potential candidate and encapsulated materials which are able to
maintain cell viability during storage and in simulated gastric juice. The four
isolates of lactic acid bacteria were examined using antagonist tested against
Escherichia coli, Staphylococcus aureus and Salmonella typhimurium. Potential
isolate obtained was AK2 with the greatest inhibition diameter 15.60 mm, 16.09
mm and 8.12 mm. AK2 has been encapsulated with 3 type materials namely
alginate (AL), alginate-skim milk-inulin (ALSI) and alginate-soy flour-inulin
(ALTI) by extrusion method. Characterization of synbiotic was performed by cell
viability test of probiotic capsules at two different temperatures; 4 oC and 27 oC
for 4 weeks of storage. Total population of bacteria after 4 weeks of storage was
relatively stable at 7-10 log CFU mL-1 in all three types of capsules. Survival of
free-cell and encapsulated cell against simulated gastric juice has been performed
with various pH; 2, 3 and 6 for 2 hours. Free-cell and AL capsule were unable to
maintain cell viability at pH 2 for 2 hours by decreasing 27.92% and 34.31%
respectively while ALSI and ALTI capsules were able to maintain cell viability by
reduction value of 12.70% and 9.44% respectively. Encapsulation double-layer of
lactic acid bacteria and prebiotic by alginate-skim milk and alginate-soy flour
were able to maintain the viability cell during storage and in simulated gastric
juice. | en_US |
