Isolasi Bakteri Endofit dari Akar Tanaman Tomat (Solanum lycopersicuml.) dan Uji Kemampuannya dalam Mendegradasi Insektisida Berbahan Aktif Karbofuran

Abstract

The study on isolation endophytic bacteria from tomato root (Solanum lycopersicum L.) and test of their ability in degrading carbofuran has been conducted. Eight bacterial isolates were obtained using Nutrient Agar (NA) medium. The result showed that all isolates were able to use carbofuran as the sole carbon source in Bushnell-Hass Broth (BHB) medium containing 2% carbofuran. Two isolates (HS06 and HS08) were selected for further test to determine their ability to degrade carbofuran using High-Performance Liquid Chromatography (HPLC). The result of the test showed that both of the isolates were able almost completely to degrade carbofuran. Isolate HS06 was able to decrease the concentration of carbofuran up to 99.87% and isolate HS08 was able to reduce carbofuran concentration up to 99.15% (original concentration of carbofuran is 175.333 ppm).This result indicates that the presence of endophytic bacteria plays an important role in degrading pesticide in plant tissue.