| dc.description.abstract | The application of probiotics in aquaculture represents an alternative way for fish
disease prevention. However, low viability and survival in application of probiotic
still being an obstacle. Encapsulation has been used to maintain probiotic viability
during storage and application. In the present study, combination of alginate-skim
milk and inulin prebiotic were used to encapsulate lactic acid bacteria PG7
isolated from digestive tract of tilapia fish (Oreochromisniloticus) from previous
study. Encapsulation had been prepared by extrusion method, then the
encapsulated bacteria were tested for its survival during storage time, simulated
gastointestinal condition, drying process, contaminant and release speed. The
result obtained indicated that encapsulation effectively protected the probiotic
from drying process, simulated gastointestinal condition and storage. The
spherical microcapsule was obtained with size of 1.91±0,27 mm and
encapsulation efficiency was 92.96%. Encapsulated probiotic could be completely
released from microspheres in simulated intestinal fluid (pH 6.8). The viability of
encapsulated probiotic retained around 7.19 log CFU/g when stored at 4 °C for 4
weeks. The survival rate of encapsulated probiotic in simulated gastric fluid (pH
2; 2 h) was 85.75%. Total yeast contamination in microsphere after 4 weeks of
storage were about 2.48-2.77 log CFU/g. This synbiotic microcapsule of isolate
PG7 showed good characteristic and efficient in maintaining viability of
bacteriain unfavourable condition, therefore it might be usefull to be applied in
aquaculture. | en_US |
